Sample Information
The Wales Cancer Bank collects frozen and paraffin embedded tumour and normal tissue, EDTA blood and serum from patients and EDTA blood from a non-blood related control (spouse, partner or friend). Samples are frozen in dry-ice cooled isopentane or liquid nitrogen and stored in freezers at -80 o C.
All frozen tissue samples have the presence of tumour verified by a pathologist and a digital image of the H & E slide is available for review.
Samples are available as detailed below:
|
Size |
Quantity |
From FROZEN blocks |
|
|
Single tumour slides |
5 micron |
5/box |
Matched tumour and normal slides |
5 micron |
5/box x 2 |
Single tumour sections (in eppendorfs) |
30 micron |
5 |
Matched tumour and normal sections (in eppendorfs) |
30 micron |
5 |
|
|
|
Extracted DNA single tumour |
1 microgram |
1 |
Extracted RNA single tumour |
5 micrograms |
1 |
Extracted DNA matched tumour and normal |
1 microgram |
1 |
Extracted RNA matched tumour and normal |
5 micrograms |
1 |
|
|
|
From PARAFFIN blocks |
|
|
Single tumour slides |
5 micron |
5/box |
Matched tumour and normal slides |
5 micron |
5/box x 2 |
|
|
|
From BLOOD |
|
|
Genomic DNA |
5 micrograms |
1 |
Whole genome amplified DNA |
5 micrograms |
1 |
Serum |
500 microlitres |
1 |
Processed material
DNA and RNA can be extracted from tissue, DNA
from blood and Tissue Microarrays can be made on request.
Frozen blocks of tissue are sectioned in a cryostat
and alternate sections used for separate extraction
of RNA and DNA. The method ensures extraction of high
quality DNA for use in techniques such as array CGH
and high quality RNA for use in expression microarray
systems.
DNA is extracted from tissue from 30 micron sections
using a Qiagen DNA kit, and RNA using a Qiagen kit or
Trizol method. Nucleic acids will be routinely supplied
as 1 microgram in 50 microlitres for DNA and 5 micrograms
in 50 microlitres for RNA.
RNA quality is assessed by 260/230 and 260/280 ratio
using a nanospectrophotometer and then subjected to
QA by Agilent Bioanalyser. Additional QA will be provided
by RT-PCR for different sizes of amplimers generated
from a housekeeping gene eg. PBGD. DNA quality is assessed
by spectrophotometry and by 1% agarose gel electrophoresis.
DNA will be extracted from blood using a Gentra. DNA
will be routinely available in 1 microgramme aliquots.
Researchers requiring different aliquot sizes should
clearly state the required amount in their application
form.
|
